10 x Column Buffer

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

10 x Column Buffer


500 ml 1 M PIPES Stock (250 mM PIPES) 40 ml of 0.5 M EGTA Stock (10 mM EGTA) 40 ml of 1 M MgSO4 Stock (5 mM MgSO4) Distilled water


  • Combine ingredients in suitable container. Add distilled water to 1800ml.

Store at 4oC

  • Adjust pH to 6.7
  • Add distilled water to 2 liters

Store at 4oC.


Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00