10 x Column Buffer¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
10 x Column Buffer
500 ml 1 M PIPES Stock (250 mM PIPES) 40 ml of 0.5 M EGTA Stock (10 mM EGTA) 40 ml of 1 M MgSO4 Stock (5 mM MgSO4) Distilled water
- Combine ingredients in suitable container. Add distilled water to 1800ml.
Store at 4oC
- Adjust pH to 6.7
- Add distilled water to 2 liters
Store at 4oC.