100 mM MgATP Stock¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
100 mM MgATP Stock
Requirements¶
ATP 1 ml of 1M MgSO4 Distilled water
Method¶
- Check the formula weight of ATP product. Calculate required quantity for 10ml of 100mM solution. Add to a suitable container.
- Add 8.5 ml distilled water
- Add 1 ml of 1M MgSO4 Stock
- Adjust pH to 7.0
- Add distilled water to final volume of 10ml
- Aliquot in 200ml quantities and store at -20oC until further use.
Store at -20oC
References¶
Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00