100 mM MgATP Stock

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

100 mM MgATP Stock

Requirements

ATP 1 ml of 1M MgSO4 Distilled water

Method

  • Check the formula weight of ATP product. Calculate required quantity for 10ml of 100mM solution. Add to a suitable container.
  • Add 8.5 ml distilled water
  • Add 1 ml of 1M MgSO4 Stock
  • Adjust pH to 7.0
  • Add distilled water to final volume of 10ml
  • Aliquot in 200ml quantities and store at -20oC until further use.

Store at -20oC

References

Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00