100mM Tris BufferΒΆ

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

Recipe for 100mM Tris Buffer

  • In a suitable container add target volume of dH20 -10% to allow for pH adjustment.

e.g. for 1L Tris, start with 900ml

  • Add 0.1211g of Tris for each 10ml dH20

e.g. for 900ml add 10.899g Tris

  • Tris solution will be basic, therefore adjust to target pH 7.0 by addition of HCl
  • Make up to final target volume with dH20