20 x Energy Regeneration System¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
20 x Energy Regeneration System
150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4) 40ml 0.5 M EGTA (2 mM EGTA) Distilled water to 10 ml
- Combine ingredients in suitable beaker. Make up to 10ml with distilled water.
- Aliquot in 100 ml containers and store at -20oC
Store at -20oC