30 mM Mg GTP-g-S

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

30 mM Mg GTP-g-S

Requirements

30 mM Mg GTP-g-S* 10 mg GTP-g-S tetralithium salt (available from Boehringer Mannheim #220 467) 18 ul 1 M MgSO4 Stock (30 mM MgSO4) 572 ul distilled water

Method

• Add 30 mM Mg GTP-g-S, 10 mg GTP-g-S tetralithium salt and 18 ul 1 M MgSO4 Stock (30 mM MgSO4) to a suitable container.
• Add 572 ul distilled water to give a final volume of 592 ul. Store at -20oC until required.

Store at -20oC

References

Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00