50 mM Tris pH 8.0 10 mM EDTA

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

50 mM Tris pH 8.0 10 mM EDTA

Requirements

50 mM 1 M Tris pH 8.0 5 ml 1 mM 0.5 M EDTA 2 ml H2O up to 100 ml

Method

• Combine Tris & EDTA to final volume of 7ml
• Add H2O up to 100 ml

Store at 4’C