Colloidal Coomassie Blue Solution

Contributed by Ian Chin-Sang, Queens University, ON, Canada

Homemade Colloidal Coomassie Blue Staining


Adjust quantities as appropriate to the following %vol:

Distilled H20|-|~650 ml Ammonium Sulfate|10%|100g Coomassie G-250|0.1%|20 ml of % solution in H20 Ortho-Phosphoric Acid|3%|30 ml Ethanol|20%|200 ml


Distilled H20 ~650 ml Ammonium Sulfate 100g Coomassie G-250 20 ml of 5% solution in H20 Ortho-Phosphoric Acid 30 ml Ethanol 200 ml


  • In a large beaker add the ingredient in the order listed. Make sure the Ammonium Sulfate is dissolved before adding the Coomassie G-250 solution to a final of 0.1%.
  • Bring final volume up to 1L with H20.
  • Store at room temperature with a tightly sealed lid.

This method is based, with permission, on an original protocol available here.