Colloidal Coomassie Blue Stain¶
Contributed by Ian Chin-Sang, Queens University, ON, Canada
Colloidal Coomassie Blue Stain for SDS-PAGE gel
- After SDS PAGE rinse Gel twice with 100 ml Distilled H20 (3 minutes each wash).
- Add Coomassie Blue working solution to Gel and stain for at least 2 hours.
- Bands should be visible without de-staining but you can de-stain with H20.
Neumann U, Khalaf H, Rimpler M Quantitation of electrophoretically separated proteins in the submicrogram range by dye elution. Electrophoresis (1994) pmid:7529171