High Salt Buffer¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
High Salt Buffer
Requirements¶
120 ml of 5 M NaCl Stock (0.6 M NaCl) 4 ml of 1 M MgSO4 Stock(4 mM MgSO4) 2 ml of 0.5 M EDTA (1mM EDTA) 10 ml of 1 M HEPES Stock (10 mM HEPES)
Method¶
- Combine ingredients in suitable container. Add distilled water to 900 ml.
- Adjust pH to 7.0
- Add distilled water to final volume of 1 liter. Store until required.
Store at 4oC
References¶
Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00