PHEM (500 mls) 2x

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

PHEM (500 mls) 2x


18.14 g Pipes 6.5 g Hepes 3.8 g EGTA 0.99 g MgSO4


  • Combine in beaker. Bring pH to pH7.0 with addition of KOH.

This method is based, with permission, on an original protocol available here.