PLC buffer for Immunoprecipitation

Contributed by Ian Chin-Sang, Queens University, ON, Canada

PLC buffer for Immunoprecipitation


50 mM Hepes 150 mM NaCl 10% glycerol 1% Triton X-100 1 mM PMSF 1.5 mM MgCl2 1 mM EGTA 10 mM NaPPi 10 mM NaF

Inhibitors: 1 mM sodium orthovanadate 10 mg/ml leupeptin 2 mM benzamidine


  • Combine the ingredients in a suitable container, and then add the inhibitors.

This method is based, with permission, on an original protocol available here.