Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
100 ml 1 M PIPES (100 mM PIPES pH= 6.9) 2 ml 1 M MgSO4, (2 mM MgSO4) 2 ml 0.5 M EGTA (1 mM EGTA,) Distilled water
- Combine ingredients in suitable container. Add distilled water to final volume of 900 ml
- Adjust pH to 6.9
- Distilled water to 1 liter. Store until required.
Store at 4oC