PMG Buffer

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

PMG Buffer


80 ml 1 M PIPES Stock (80 mM PIPES) 2 ml 1 M MgSO4 Stock, (2 mM MgSO4) 2 ml 0.5 M EGTA Stock (1 mM EGTA,) 600 ml Glycerol Distilled water


  • Combine ingredients. Add distilled water to 900 ml, mix well
  • Adjust pH to 6.9
  • Add distilled water to 1 liter. Store for later use.

Store at 4oC


Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00