Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
80 ml 1 M PIPES Stock (80 mM PIPES) 2 ml 1 M MgSO4 Stock, (2 mM MgSO4) 2 ml 0.5 M EGTA Stock (1 mM EGTA,) 600 ml Glycerol Distilled water
- Combine ingredients. Add distilled water to 900 ml, mix well
- Adjust pH to 6.9
- Add distilled water to 1 liter. Store for later use.
Store at 4oC