Cell Culture Freezing Media¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
Media for freezing of cells for culture.
700ml RPMI (2mM L-Glutamine) 200ml FCS 100ml DMSO
- Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal calf serum (FCS) through a 0.22µm filter
- Add 100ml sterile dimethyl sulfoxide (DMSO)
Do not filter DMSO, it will dissolve the cellulose acetate membrane.
- Aliquot into tubes as appropriate
Store the tubes at -80oC for up to one year.