Cell Culture Freezing Media

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

Media for freezing of cells for culture.


700ml RPMI (2mM L-Glutamine) 200ml FCS 100ml DMSO


  • Filter sterilize 700ml of RPMI-1640 (2mM L-Glutamine) and 200ml fetal calf serum (FCS) through a 0.22µm filter
  • Add 100ml sterile dimethyl sulfoxide (DMSO)

Do not filter DMSO, it will dissolve the cellulose acetate membrane.

  • Aliquot into tubes as appropriate

Store the tubes at -80oC for up to one year.