Acetic alcohol fixation¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
Fixative for smears, cytospin preparations and frozen sections
- Add 3ml glacial acetic acid to 100ml 95% methanol
- Fix section in solution for 1 minute
- Rinse section in distilled water prior to staining
This method is based, with permission, on an original protocol available here.