Acetic alcohol fixationΒΆ

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

Fixative for smears, cytospin preparations and frozen sections

  • Add 3ml glacial acetic acid to 100ml 95% methanol
  • Fix section in solution for 1 minute
  • Rinse section in distilled water prior to staining

This method is based, with permission, on an original protocol available here.