DAPI counterstain adherent cells/sectionsΒΆ

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

DAPI counterstain of adherent cells or tissue sections on slides

  • Perform all fixation, permeabilization and primary staining of your sample before applying the DAPI counterstain. Fixation or permeabilization is not required to stain with DAPI.
  • Wash and equilibrate section in PBS for 5 minutes
  • Dilute stock DAPI to 300nM concentration in PBS
  • Add 300ul of diluted DAPI per spot to the coverslip. Ensure cells/section is completely covered by the droplet.
  • Incubate at room temperature for 1-5minutes
  • Place sample in PBS bath, washing 3x for 5 minutes then replacing the PBS.
  • Add coverslip with appropriate mounting medium