BrdU incorporation for raft cultures¶
Contributed by Matt Lewis <email@example.com>
BrdU incorporation for raft cultures
- Prepare stocks of BrdU at 10mg/ml in H2O, filter sterilise and store in working aliquots (eg. 200 microlitres) at –20C.
- Incubate rafts with BrdU (100 microgram per ml) for 12 hours prior to harvesting.
- Detect incorporated BrdU via antibody staining (Zymed 18-0103) using standard immunohistochemical techniques
High temperature antigen retrieval is required to open up the DNA
This method is based, with permission, on an original protocol available here.