Chromosome FISHΒΆ

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

DAPI counterstain for chromosome FISH analysis

  • After FISH sample preparation briefly rinse sample in distilled water to remove residual salts
  • Dilute stock DAPI to a concentration of 30nM in PBS
  • Add 300ul of diluted DAPI solution directly onto the sample, ensuring sample is covered equally
  • Incubate at room temperature for 30 minutes in the dark
  • Rinse specimen with distilled water or PBS to remove DAPI solution
  • Blot edge of slide with tissue to remove excess water/solution from the side
  • Mount slide with appropriate slide mountant, nail polish or wax.