Molecular biology

Molecular biology protocols.

• 5X Long PCR Buffer
• 6X DNA Loading Buffer for Agarose
• Agarose gel electrophoresis
• APES treatment of slides
• Bacterial colony screening by PCR
• Bacterial mediated RNAi
• C. elegans microinjection
• C. elegans Single Worm PCR
• C. elegans total genomic DNA preparation
• Determining DNA concentrations for ligations
• DNA Extraction: Chelex
• DNA extraction from agarose gels (Dialysis-tubing)
• DNA extraction from agarose gels (Paper-strip)
• DNA extraction from agarose gels (Spin-column)
• DNA Extraction from Cheek Cells
• DNA fragment isolation
• DNA ligation
• Excision of Clones as pBluescript from lZAPII
• In situ hybridisation to alpha satellite sequences
• Lacritin Stimulated Cell Proliferation Assay
• Measuring DNA Contour Lengths with ImageJ
• Metabolite extraction from supernatant cells
• PCR mutagenesis
• PCR product “clean-up” (SAP/EXO)
• Phosphatase Removal of PO4 groups from DNA
• Preparation of Competent XLI-Blue MRF’ Cells
• Preparation of Sonicated Salmon Sperm DNA
• Proteinase K treatment
• Retroviral infection of PHKs
• Retrovirus titration
• RNA-isolation (TRIZOL method)
• T-A cloning Vectors
• Transformation of plasmid DNA to E. Coli
• Transformation of XLI-Blue MRF’ Competent Cells