Welcome to The Open Lab Book¶
The OLB is a collection of open, freely-available lab protocols collected, with permission, from across the internet. The list has been available in various formats and on various sites over that time, but finally finds a home here.
Authors are credited where known and with homepage links where available. Contributions of new protocols or amendments or corrections to existing ones are most welcome and can be made via the github repository.
All content is licensed under the Creative Commons Attribution-ShareAlike 2.5 (CC BY-SA 2.5).
You can also download the complete book as a PDF.
Contents¶
- Biochemistry
- Buffers & Solutions
- 0.1 M Glycine-HCl, pH 2.7
- 0.5 M EDTA
- 0.5 M EGTA Stock
- 0.5 M Na2CO3
- 1 M HEPES, pH = 7.0
- 1 M KCl Stock
- 1 M PIPES, pH = 6.9
- 10% Ammonium persulphate solution
- 10 M NaOH
- 10 x Column Buffer
- 100 mM MgATP Stock
- 100 mM MgGTP
- 100 mM Sodium orthovanadate
- 100mM Tris Buffer
- 10x Tris Buffer
- 150 mM Mg AMP-PNP
- 1M dithiothreitol (DTT)
- 1M MgSO4
- 20 x Energy Regeneration System
- 2X Rapid Ligation Buffer
- 3 M KI
- 30 mM Mg GTP-g-S
- 30 x AlCl3
- 30 x NaF
- 3.6 mM Brefeldin A
- 5 M NaCl
- 50 mM Tris pH 8.0 10 mM EDTA
- 5x Western blot loading buffer
- 8% Paraformaldehyde for Immunofluorescence
- Colloidal Coomassie Blue Solution
- Colloidal Coomassie Blue Stain
- High Salt Buffer
- Homogenization Buffer
- Isolation Buffer
- PBS (1x in 1L)
- PBS (5x in 500 mls)
- PHEM (500 mls) 2x
- PLC buffer for Immunoprecipitation
- PM Buffer
- PMG Buffer
- Sodium Citrate Buffer
- Cell biology
- Histology
- Acetic alcohol fixation
- Antibody diluent for IHC
- Bouin’s fluid fixation
- C. Elegans Embryo Staining
- Carnoy’s fluid fixation
- Cresyl Violet Staining (Nissl Staining)
- Dewaxing Paraffin Sections
- Ethanol fixation for flow cytometry
- Fixation with paraformaldehyde
- Fixing/pelleting chromatin for immunofluorescence
- Formaldehyde fixation for flow cytometry
- Formol sublimate fixation
- Haemotoxylin and Eosin Staining
- Helly’s fluid fixation
- Immunohistochemistry (cultured cells/monolayers)
- Immunohistochemistry with TSA
- Immunohistochemistry
- Immunostaining of Cells Adherent to Coverslips
- Immunostaining of Paraffin Sections
- Luxol Fast Blue staining
- Michel’s fixative for immunofluorescence
- Mounting Media
- Neuron Recovery of Neurobiotin/biocytin cells
- Neutral buffered formalin fixation
- Nickel DAB (N-DAB) Labeling
- Preparation for microdissection (Paraffin/Frozen)
- Silane/acetone silanized slides
- Tissue Preparation for Vibratome Sectioning
- Zenker’s fluid fixation
- Imaging
- Immunology
- Miscellaneous
- Automatic unzip/untar using correct tool
- BrdU incorporation for raft cultures
- Cell Adhesion
- Cells in suspension
- Chemiluminescent Detection
- Chrome Alum Gelatin Slide Coating
- Chromosome FISH
- Collagen gel containing 3T3 fibroblasts
- Cosmid / Low Copy Plasmid Prep
- Cresyl Violet Staining (Nissl Staining)
- Crude Depletion Conditions for XKCM1
- CSF Extract Prep for Spindle Assembly
- EMS Mutagenesis
- Endotoxin Assay
- Frozen Yeast Competent Cells
- Heparan Sulfate Chain Analysis
- Heparanase Western Blotting
- HSS Depletion Conditions for XKCM1
- Isolation of LDL From Human Plasma
- L B Broth
- Lacritin Calcium Signaling Assay
- Lacritin IP3 Assay
- Lacritin PKCa Signaling Assay
- LB Agar Plates
- M9 Plates -Leu
- Microtubule Spindowns from Extracts
- Organotypic raft culture of PHKs
- Preparation for microdissection (Polyester)
- Raft culture media
- Rotary Shadowing
- Silica-based Plasmid Miniprep (vacuum manifold)
- Silica Suspension for plasmid recovery
- Tear Collection Method
- TMP UV Integration
- Transformation of Frozen Saccharomyces cerevisiae
- Using a Gel Doc system
- Valap
- X-gal overlay assay for Yeast plates
- Zymolyase plasmid recovery from Yeast
- Model organisms
- Molecular biology
- 5X Long PCR Buffer
- 6X DNA Loading Buffer for Agarose
- Agarose gel electrophoresis
- APES treatment of slides
- Bacterial colony screening by PCR
- Bacterial mediated RNAi
- C. elegans microinjection
- C. elegans Single Worm PCR
- C. elegans total genomic DNA preparation
- Determining DNA concentrations for ligations
- DNA Extraction: Chelex
- DNA extraction from agarose gels (Dialysis-tubing)
- DNA extraction from agarose gels (Paper-strip)
- DNA extraction from agarose gels (Spin-column)
- DNA Extraction from Cheek Cells
- DNA fragment isolation
- DNA ligation
- Excision of Clones as pBluescript from lZAPII
- In situ hybridisation to alpha satellite sequences
- Lacritin Stimulated Cell Proliferation Assay
- Measuring DNA Contour Lengths with ImageJ
- Metabolite extraction from supernatant cells
- PCR mutagenesis
- PCR product “clean-up” (SAP/EXO)
- Phosphatase Removal of PO4 groups from DNA
- Preparation of Competent XLI-Blue MRF’ Cells
- Preparation of Sonicated Salmon Sperm DNA
- Proteinase K treatment
- Retroviral infection of PHKs
- Retrovirus titration
- RNA-isolation (TRIZOL method)
- T-A cloning Vectors
- Transformation of plasmid DNA to E. Coli
- Transformation of XLI-Blue MRF’ Competent Cells