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  • Biochemistry
  • Buffers & Solutions
    • 0.1 M Glycine-HCl, pH 2.7
    • 0.5 M EDTA
    • 0.5 M EGTA Stock
    • 0.5 M Na2CO3
    • 1 M HEPES, pH = 7.0
    • 1 M KCl Stock
    • 1 M PIPES, pH = 6.9
    • 10% Ammonium persulphate solution
    • 10 M NaOH
    • 10 x Column Buffer
    • 100 mM MgATP Stock
    • 100 mM MgGTP
    • 100 mM Sodium orthovanadate
    • 100mM Tris Buffer
    • 10x Tris Buffer
    • 150 mM Mg AMP-PNP
    • 1M dithiothreitol (DTT)
    • 1M MgSO4
    • 20 x Energy Regeneration System
    • 2X Rapid Ligation Buffer
    • 3 M KI
    • 30 mM Mg GTP-g-S
    • 30 x AlCl3
    • 30 x NaF
    • 3.6 mM Brefeldin A
    • 5 M NaCl
    • 50 mM Tris pH 8.0 10 mM EDTA
    • 5x Western blot loading buffer
    • 8% Paraformaldehyde for Immunofluorescence
    • Colloidal Coomassie Blue Solution
    • Colloidal Coomassie Blue Stain
    • High Salt Buffer
    • Homogenization Buffer
    • Isolation Buffer
    • PBS (1x in 1L)
    • PBS (5x in 500 mls)
    • PHEM (500 mls) 2x
    • PLC buffer for Immunoprecipitation
    • PM Buffer
    • PMG Buffer
    • Sodium Citrate Buffer
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Buffers & SolutionsΒΆ

Buffer and solution protocols.

  • 0.1 M Glycine-HCl, pH 2.7
  • 0.5 M EDTA
  • 0.5 M EGTA Stock
  • 0.5 M Na2CO3
  • 1 M HEPES, pH = 7.0
  • 1 M KCl Stock
  • 1 M PIPES, pH = 6.9
  • 10% Ammonium persulphate solution
  • 10 M NaOH
  • 10 x Column Buffer
  • 100 mM MgATP Stock
  • 100 mM MgGTP
  • 100 mM Sodium orthovanadate
  • 100mM Tris Buffer
  • 10x Tris Buffer
  • 150 mM Mg AMP-PNP
  • 1M dithiothreitol (DTT)
  • 1M MgSO4
  • 20 x Energy Regeneration System
  • 2X Rapid Ligation Buffer
  • 3 M KI
  • 30 mM Mg GTP-g-S
  • 30 x AlCl3
  • 30 x NaF
  • 3.6 mM Brefeldin A
  • 5 M NaCl
  • 50 mM Tris pH 8.0 10 mM EDTA
  • 5x Western blot loading buffer
  • 8% Paraformaldehyde for Immunofluorescence
  • Colloidal Coomassie Blue Solution
  • Colloidal Coomassie Blue Stain
  • High Salt Buffer
  • Homogenization Buffer
  • Isolation Buffer
  • PBS (1x in 1L)
  • PBS (5x in 500 mls)
  • PHEM (500 mls) 2x
  • PLC buffer for Immunoprecipitation
  • PM Buffer
  • PMG Buffer
  • Sodium Citrate Buffer
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© Copyright 2014, Martin Fitzpatrick Revision 339cd45d.

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