Formol sublimate fixation

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

Fixation in formol sublimate

• In a fume hood pour 100ml formalin (40% aqueous solution of formaldehyde) into a suitable container
• Add 900ml saturated aqueous mercuric chloride
• Submerge tissue and fix for 4-6 hours
• Remove fixed tissue and transfer to 80% alcohol to store

This method is based, with permission, on an original protocol available here.