Zenker’s fluid fixation

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

Fixation with Zenker’s fluid

  • Add 950ml distilled water to a suitable container
  • Add 25g potassium dichromate
  • Add 50g mercuric chloride
  • Add 50g glacial acetic acid
  • Mix thoroughly to dissolve
  • Fix samples for 4-24 hours
  • Following fixation wash samples overnight in running tap water prior to processing

This method is based, with permission, on an original protocol available here.