L B Broth¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
L B Broth
1 liter of ddH2O 10 grams Bacto-tryptone 5 grams Bacto-yeast Extract 5 grams NaCl
- In 1 liter of ddH2O combine 10 grams Bacto-tryptone, 5 grams Bacto-yeast Extract and 5 grams NaCl
- Stir until dissolved.
- Autoclave for at least 15min @120C
- Once cooled below 55C add antibiotic of choice and store at 4C. Use within 2 weeks
This method is based, with permission, on an original protocol available here.