L B Broth

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

L B Broth


1 liter of ddH2O 10 grams Bacto-tryptone 5 grams Bacto-yeast Extract 5 grams NaCl


  • In 1 liter of ddH2O combine 10 grams Bacto-tryptone, 5 grams Bacto-yeast Extract and 5 grams NaCl
  • Stir until dissolved.
  • Autoclave for at least 15min @120C
  • Once cooled below 55C add antibiotic of choice and store at 4C. Use within 2 weeks

This method is based, with permission, on an original protocol available here.