100mM Tris BufferΒΆ
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
Recipe for 100mM Tris Buffer
- In a suitable container add target volume of dH20 -10% to allow for pH adjustment.
e.g. for 1L Tris, start with 900ml
- Add 0.1211g of Tris for each 10ml dH20
e.g. for 900ml add 10.899g Tris
- Tris solution will be basic, therefore adjust to target pH 7.0 by addition of HCl
- Make up to final target volume with dH20