8% Paraformaldehyde for Immunofluorescence

Contributed by Luke Hammond, QBI, The University of Queensland, Australia

8% Paraformaldehyde for Immunofluorescence

  • Add 16g paraformaldehyde to approximately 160ml water in fume hood.
  • Heating on setting 4.5 whilst stirring to 50ºC.
  • Once temperature reaches 50ºC, 1M NaOH until the paraformaldehyde is just dissolved.
  • Make up to final volume of 200ml with water, pH to 7.2-7.4.
  • Filter with 0.22µm filter.
  • Aliquot in 5ml tubes, label and freeze.

This method is based, with permission, on an original protocol available here.