Isolation Buffer

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

Isolation Buffer

Requirements

20 ml 5 M NaCl Stock (0.1 M NaCl) 4 ml of 1 M MgSO4 Stock (4 mM MgSO4) 2 ml of 0.5 M EDTA (1mM EDTA) 10 ml of 1 M HEPES Stock (10 mM HEPES) Distilled water

Method

• Combine ingredients in suitable container. Add distilled water to 900 ml.
• Adjust pH to 7.0
• Add further distilled water to final volume of 1 litre. Store until required.

Store at 4oC

References

Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00