20 x Energy Regeneration System

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

20 x Energy Regeneration System

Requirements

150 mM creatine phosphate (Boehringer #127574) 2 ml of 100 mM MgATP Stock (20 mM ATP, 20 mM MgSO4) 40ml 0.5 M EGTA (2 mM EGTA) Distilled water to 10 ml

Method

• Combine ingredients in suitable beaker. Make up to 10ml with distilled water.
• Aliquot in 100 ml containers and store at -20oC

Store at -20oC

References

Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00