Colloidal Coomassie Blue Stain

Contributed by Ian Chin-Sang, Queens University, ON, Canada

Colloidal Coomassie Blue Stain for SDS-PAGE gel

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  • After SDS PAGE rinse Gel twice with 100 ml Distilled H20 (3 minutes each wash).
  • Add Coomassie Blue working solution to Gel and stain for at least 2 hours.
  • Bands should be visible without de-staining but you can de-stain with H20.