PM Buffer

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

PM Buffer

Requirements

100 ml 1 M PIPES (100 mM PIPES pH= 6.9) 2 ml 1 M MgSO4, (2 mM MgSO4) 2 ml 0.5 M EGTA (1 mM EGTA,) Distilled water

Method

  • Combine ingredients in suitable container. Add distilled water to final volume of 900 ml
  • Adjust pH to 6.9
  • Distilled water to 1 liter. Store until required.

Store at 4oC

References

Clare M. Waterman-Storer Microtubule/Organelle Motility Assays (2001) 10.1002/0471143030.cb1301s00