Antibody diluent for IHC

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Antibody diluent for IHC

Make sure you use good quality crystalline BSA e.g. fatty acid free from sigma.


NaCl Proclin 300 RIA/IHC grade BSA 1M Tris-HCl pH 7.6


  • Add the following items to 90mls ultra pure water
  1. 870mg NaCl
  2. 50ul Proclin 300
  3. 1g BSA
  4. 5mls 1M Tris-HCL pH 7.6
  • Make up to 100mls total volume with ultra pure water, stir until completely dissolved.
  • Filter the solution through a sterile 0.2um filter, aliquot and store at -20C
  • Once thawed solution is stable at 4C for at least a week.

It can also be used for long term storage (>6m) of antibody working solutions.