Silane/acetone silanized slidesΒΆ

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

Silanized (3-aminopropyltriethoxysilane) slides to prevent sections from detaching.

  • Place slides in 2% silane/acetone solution for 1 minute
  • Transfer slides to 100% acetone for 1 minute
  • Transfer slides to double distilled water. Agitate for 1 minute
  • Replace double distilled water, and wash repeat x2 for 1 minute
  • Leave slides to air dry overnight at room temperature
  • Slides may be kept indefinitely covered in slide box until ready for use