Dewaxing Paraffin Sections

Contributed by Luke Hammond, QBI, The University of Queensland, Australia

Dewaxing Paraffin Sections Sections must be free of wax to allow aqueous solutions to penetrate.

Cryostat and free floating sections may need defatting prior to immunhistochemical staining. This must be optimised by the user


  • Xylene x2 (2-3mins)
  • Absolute ethanol x2 (2-3mins)
  • Water wash.

This method is based, with permission, on an original protocol available here.