100 mM Sodium orthovanadate¶
Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom
100 mM Sodium orthovanadate
1.839 g Sodium orthovanadate 8ml distilled water to 8 ml
- Add 1.839 g Sodium orthovanadate to a screw cap tube (falcon)
- Make up to 8ml with distilled water.
- Adjust pH to 10
- If solution is yellow, place in boiling water until clear. Re-check pH and repeat as necessary.
- Adjust to final concentration by checking A265 nm, ext. coeff.= 2925M-1cm-1and adding distilled water as needed. Store until required.
Store at -20oC