PBS (5x in 500 mls)

Contributed by Martin Fitzpatrick, University of Birmingham, United Kingdom

PBS (5x in 500 mls)



20.45 g NaCl 0.465 g KCl 10.142 g Na2HPO4*7 H2O 0.545 g KH2PO4


  • Add 500ml distilled water to a suitable container.
  • Add ingredients to water and pH to 7.2

This method is based, with permission, on an original protocol available here.